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Baboon, Dog, Cat, Rabbit, Rat, Horse

2°C to 8°C  (≤ 30 days)

For long-term storage (> 30 days), aliquot and store at ≤ –20°C. Avoid repeated freeze-thaw.

For Research Use Only. Not for use in diagnostic procedures

Highly purified human C6 was isolated from normal serum and used to immunize goats. The anti-human C6 polyclonal antisera was tested against normal human plasma by double immunodiffusion, one-dimensional immunoelectrophoresis, quantitative radial immunodiffusion, and quantitative rocket immunoelectrophoresis. The antiserum was determined to be monospecific for C6 at varying concentrations.

C6 is a plasma glycoprotein which is present in normal human serum/plasma at approximately 60 μg/mL. Primarily synthesized in the liver, C6 is a single-chain protein with a molecular weight of approximately 105 kD. With activation of the classical or alternative pathways of the complement system, C5 convertase cleaves the C5 chain into C5a and C5b. C5b remains bound to the convertase and will combine with C6 to form a C5b,6 complex. This complex normally interacts with C7 to form C5b,6,7 that becomes partially embedded in the target cell outer membrane.

Applications of the C6 polyclonal antisera have been evaluated by various research facilities, and include Western Blot, 1 IHC, 2,3 and ELISA. 4