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Concentration
5-10u/μl
5'…GDGCH↓C…3'
3'…C↑HCGDG…5'

Reaction Conditions 
1.5X Buffer UB 
37.5mM Tris-acetate (pH 7.6 at 30°C), 15mM Mg-acetate, 150mM K-acetate, 10.5 mM 2-mercaptoethanol and 75μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
80°C for 20 minutes.

Ligation / Recutting Assay 
After 5-fold overdigestion with MhlI, more than 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 5u of MhlI for 16 hours at 37°C.
Supplied with 10X Buffer UB and Viva Buffer A 
* High enzyme concentration may result in Star Activity.

Ordering Information

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Manual
MhlI {SduI}