亚洲欧美日韩中文在线制服,中文在线最新版天堂,制服 丝袜 国产,亚洲伊人久久大香线蕉,亚洲精品乱码久久久久久蜜桃

Concentration
5-20u/μl
5'…GTY↓RAC…3' 
3'…CAR↑YTG…5'

Reaction Conditions 
0.5X Buffer UB
12.5mM Tris-acetate (pH7.6 at 30°C), 5mM Mg-acetate, 50mM K-acetate, 3.5mM 2-mercaptoethanol and 25μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 10-fold overdigestion with HindIl, more than 60% of the DNA fragments can be ligated and recut. In the presence of 10% PEG, ligation is better.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 20u of HindII for 16 hours at 37°C
Supplied with 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

Downloads
Manual
HindII