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Concentration
5-10u/μl 
5'…C^↓CGG…3'
3'…GGC_↑C…5'

Reaction Conditions 
1X Buffer V1
10mM Tris-HCl (pH 7.5 at 30°C), 10mM MgCl₂ and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 100μg/ ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
None.

Ligation / Recutting Assay 
After 5-fold overdigestion with HpaII, more than 95% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of HpaII for 16 hours at 37°C.
Supplied with 10X Buffer V1, 10X Buffer UB and Viva Buffer A. (Diluent)
Blocked by CpG methylation.

Ordering Information

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Manual
HpaII

Publication
This Product Has Been Used In:
Ruzilawati, A.B., Mohm Suhaimi, A.W., Gan, S.H. (2007) . Genetic polymorphisms of CYP3A4: CYP3A4?18 allele is found in five healthy Malaysian subjects. Clinica Chimica Acta. 383(1-2) pp.158-162