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Concentration
10-30u/μl 
5'…RGCGC↓Y…3' 
3'…Y↑CGCGR…5'

Reaction Conditions 
1X Buffer V5
30mM Tris-acetate (pH 7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 65°C.

Storage Buffer
10mM KH₂PO₄ (pH 7.2), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
None.

Ligation / Recutting Assay 
After 20-fold overdigestion with BstH2I, more than 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of BstH2I for 16 hours at 65°C. 
Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent) 
*High enzyme concentration may result in Star Activity.

Ordering Information

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Manual
BstH2I {HaeII}