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Concentration
2-10u/μl 
5'…CG↓CG…3'
3'…GC↑GC…5'

Reaction Conditions 
1X Buffer V5
30mM Tris-acetate (pH 7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 60°C.

Storage Buffer
20mM Tris-HCl (pH 7.5), 300mM NaCl, 10mM MgCl₂ 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
None.

Ligation / Recutting Assay 
After 10-fold overdigestion with BstFNI, 95% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of BstFNI for 16 hours at 60°C. 
Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent) 
*Blocked by CpG methylation

Ordering Information

Downloads
Manual
BstFNI {FnuDII}