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Concentration
2-10u/μl
5'…GG↓CC…3' 
3'…CC↑GG…5'

Reaction Conditions 
1X Buffer V4
10mM Tris-HCl (pH 8.5 at 30°C), 10mM MgCl₂, 100mM KCl, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 0.15% Triton X-100, 500μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
80°C for 20 minutes.

Ligation / Recutting Assay 
After 6-fold overdigestion with BsnI, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of BsnI for 16 hours at 37°C.
Supplied with 10X Buffer V4, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

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Manual
BsnI {HaeIII}

Publication
This Product Has Been Used In:
Chemkhi, J., Souguir, H., Ali, I.B.H., Driss, M., Guizani, I., Gouerbouj, S. (2015) Natural infection of Algerian hedgehog, Atelerix algirus (Lereboullet1842) with Leishmania parasites in Tunisia. Acta Tropica. 150. Pp42-51.