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Concentration
10- 30u/μl 
5'…CC↓TNAGG…3' 
3'…GGANT↑CC…5'

Reaction Conditions 
1X Buffer V5
30mM Tris-acetate (pH 7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM KH₂PO₄ (pH 7.4), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
None.

Ligation / Recutting Assay 
After 10-fold overdigestion with Bse21I, 50% of the DNA fragments can be ligated by using high concentration of T4 DNA ligase and of these more than 90% can be recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 20u of Bse21I for 16 hours at 37°C. 
Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

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Manual
Bse21 I {SauI}