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Concentration
1-10u/μl 
5'…GTA↓TAC…3' 
3'…CAT↑ATG…5'

Reaction Conditions 
1X Buffer V4 
0mM Tris-HCl (pH 8.5 at 30°C), 10mM MgCl2, 100mM KCl, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 100μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
None.

Ligation / Recutting Assay 
After 5-fold overdigestion with BssNAI, more than 90% of the DNA pBR322 can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of BssNAI for for 16 hours at 37°C.
Supplied with 10X Buffer V4, 10X Buffer UB and Viva Buffer A. (Diluent)
* High enzyme concentration may result in Star Activity.

Ordering Information

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Manual
BssNAI {SnaI}