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Concentration
1-5u/μl 
5'…GCCNNNN↓NGGC…3' 
3'…CGGN↑NNNNCCG…5'

Reaction Conditions 
1X Buffer Bbv12I 
20mM Tris-HCl (pH8.5), 10mM MgCl2, 20mM NaCl, and 1mM DTT. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
None.

Ligation / Recutting Assay 
After 5-fold overdigestion with Bbv12I, more than 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 5u of Bbv12I for 16 hours at 37°C. 
Supplied with 10X Buffer V3, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

Downloads
Manual
Bbv12l {HgiAI}