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Concentration
5-20u/μl 
5'…G↓GWCC…3' 
3'…CCWG↑G…5'

Reaction Conditions 
1X Buffer V3 
50mM Tris-HCl (pH 7.5 at 30°C), 10mM MgCl₂ , 100mM NaCl, and 100μg /ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 100μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 5-fold overdigestion with Bme18I, more than 90% of the DNA fragments can be ligated.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of Bme18I for 16 hours at 37°C. 
Supplied with 10X Buffer V3, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

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Manual
Bme18I {AvaII}