亚洲综合熟女久久久30p,亚洲伊人久久综合成人,一本色道久久88综合日韩精品

您好，歡迎來到拜力生物網(wǎng)站！請登錄注冊

我的訂單 | 聯(lián)系我們 | 在線客服QQ：3012681483 | 客服電話：400-968-7988 | 工作時間：08:30-17:30

主營產(chǎn)品： ELISA試劑盒、抗體、補(bǔ)體血清、兔補(bǔ)體、干擾素、小鼠測溫儀、進(jìn)口試劑、Quidel代理、Swant代理、PBL Assay Science代理

網(wǎng)站首頁產(chǎn)品中心代理品牌新聞資訊資源中心關(guān)于我們

所在位置：首頁 > 產(chǎn)品中心 > abmgood > abmgood產(chǎn)品

Immortalized Human Fallopian Tube Secretory Epithelial Cells (FT33-shp53-R24C)

訂購信息

上海拜力生物科技公司

Tel:400-968-7988 021-33779008

Immortalized Human Fallopian Tube Secretory Epithelial Cells (FT33-shp53-R24C)

品牌：Abmgood

貨號：T0609

規(guī)格：

貨期：

應(yīng)用：

咨詢客服

立即購買加入購物車

Immortalized Human Fallopian Tube Secretory Epithelial Cells (FT33-shp53-R24C)

商品詳情參考文獻(xiàn) 相關(guān)資料

	Print Version
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Fimbriae of fallopian tube
Growth Properties	Adherent
Morphology	Cobblestone-like
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers	PAX8, CK7
Applications	For Research Use Only
Immortalization Method	Serial passaging and transduction with retroviruses stably expressing hTERT, p53 shRNA and CDK4^R24C mutant gene
Description	The fallopian tube epithelium is lined by both ciliated and secretory epithelial cells. Recently, the fallopian tube epithelial secretory epithelial cells (FTSEC) are proposed to be the precursor tissue for high grade serous ovarian cancer (HGSC), the most lethal gynecological malignancies. The Immortalized Human Fallopian Tube Epithelial Secretory Cells (FT33-shp53-R24C) maintains its Müllerian phenotype and continues to express lineage-specific markers PAX8 and CK7, thus providing a valuable tool for studies of normal fallopian tube epithelium and early-stage development of HGSC.
Procedure Overview
Image
Image
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow IV medium available from ABM (TM004). To make the complete growth medium, add the following components to the base medium: Ultroser G (Pall Corporation) at final concentration of 2%, and antibiotics (50 μg/ml gentamicin (G272) or 1% Penicillin/Streptomycin (G255)). Atmosphere: air: 95%, CO2: 5%; Temperature: 37.0°C.
Preservation	1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control	1) Western blot and RT-PCR were used to evaluate the hTERT and CDK4 transgene expression; 2) CDK4 functional studies was performed to assess the hyperphosphorylation of pRb at Ser780; 3) Immunofluorescent staining was used to determine PAX8 expression.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.