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Product Description

ATA-Phe-Pro-Arg-CMK

ATA-FFRCK (Na-(acetylthio) acetyl-D-Phe-Phe-Arg-CH2Cl) and ATA-FPRCK (Na-(acetylthio)acetyl-D-Phe-Pro-Arg-CHCl2) are active-site specific labeling reagents for serine proteases. They are derivatized from irreversible peptide chloromethyl ketones and facilitate incorporation of a thioester moiety via alkylation of the catalytic site Histidine residue. Liberation of the free thiol group is accomplished by gentle treatment with hydroxylamine (NH2OH) following irreversible incorporation into the enzyme catalytic site.