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上海拜力生物科技公司
Tel:400-968-7988    021-33779008
CD4 Antibody (PE)
品牌：Abbexa
貨號(hào)：abx134642
規(guī)格：50 Assays
貨期：
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CD4 Antibody (PE)
商品詳情 參考文獻(xiàn) 相關(guān)資料
Mouse monoclonal antibody to CD4 (PE).

Target CD4
Reactivity Human
Host Mouse
Clonality Monoclonal
Tested Applications IF/ICC, FCM
Purification The antibody was purified by affinity chromatography.
Form Liquid
Isotype IgG2a
Conjugation PE
Specificity Recognises human CD4.
Storage Shipped and store at 4°C for one year. Do not freeze.
Swiss Prot P01730
GeneID 920
Gene Symbol CD4
Buffer PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide.
Directions for use 1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of a 5 ml tube.
2. Add 10 μl labeled antibody to the bottom of the flow tube mixing with the whole blood; incubate for 20 minutes at room temperature away from light.
3. Add 2 ml RBC lysis buffer; incubate for 10 minutes away from light after mixing; dissolve red blood cells.
4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant.
5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant.
6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
Availability Shipped within 5-10 working days.
Note This product is for research use only.
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