品牌：Emfret
貨號(hào)：M042-1
品名：Emfret FITC-labeled Rat Anti-Mouse GPIbα (CD42b)
規(guī)格：1.5 ml / 300 tests
克隆：Xia.G7 / Rat (Wistar) IgG1
包含：FITC-labeled immunoglobulin in 20 mM Tris buffer with 137 mM NaCl, 0.5%
BSA and 0.09% (w/v) sodium azide
特異性：
The Xia.G7 antibody reacts with mouse GPIbα (CD42b), a platelet/megakaryocytespecific 150 kDa polypeptide that is disulfide-linked with GPIbβ (24 kDa) in the membrane. GPIb is
part of the GPIb-V-IX complex, the platelet receptor for von Willebrand factor (vWf)1,2. Xia.G7 binds to
the 130 kDa extracellular domain of GPIbα
3
, which is proteolytically released during platelet activation.
Xia.G7 recognizes the membrane bound and the soluble form of GPIbα
3
.
用途：
The antibody preparation is optimized for flow cytometric applications: Use 5 μl to stain ~106
platelets or ~0.5 x 106 cells in a recommended volume of 25 μl. Incubate for 15 minutes at room
temperature, stop reaction by addition of 400 μl PBS and analyze samples within 30 minutes. For
immunofluorescent staining of acetone-fixed frozen sections, the appropriate dilution must be
determined individually.
制備和儲(chǔ)存：
The antibody was purified from hybridoma cell culture supernatant by
Protein G-Sepharose chromatography. The antibody was conjugated with FITC under optimum
conditions. The solution is free of unbound FITC. Store product undiluted at 4°C and avoid prolonged
exposure to light. Stable for one year from date of shipment. Do not freeze.
注意：
Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations
containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may
be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free
buffer.
注：僅供研究使用，不用于診斷或治療用途。
