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Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM175; H3K27me3, Histone H3 (trimethyl K27); ChIP Validated
品牌：RevMAb
貨號：31-1062-00
規(guī)格：100 ug
貨期：電詢
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Product Details
Product Name Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody [RM175]
Product Description Rabbit monoclonal to Trimethylated Histone H3 Lysine 27, H3K27me3, Histone H3 (trimethyl K27)
Catalog No. 31-1062-00
Clone Name RM175
Product Data Sheet Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody Data Sheet RM175
Specificity RM175 reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity non-modified Lysine 27, monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in histone H3.
Immunogen A trimethyl-peptide corresponding to trimethyl-Histone H3 (Lys27).
Application Western Blot (WB)
Immunohistochemistry (IHC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 1 ug/mL – 2 ug/mL;
IHC: 0.5 ug/mL – 2 ug/mL;
ELISA: 0.5ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords
.
Product Images
RM175

RM175 specifically reacts to Histone H3
trimethylated at Lysine 27 (K27me3). No
cross reactivity with non-modified Lysine 27
 (K27 Ctrl), monomethylated Lysine 27
(K27me1) or dimethylated Lysine 27 (K27me2),
or other methylations in Histone H3.

Western

Western Blot of recombinant histone H3.3 (1) and
 acid extracts of HeLa cells (2), using RM175 at
1 ug/mL, showed a band of histone H3 trimethyl-
ated at Lysine 27 (K27me3) in HeLa cells.

Immunohistochemistry

Immunohistochemistry staining of HepG2 cells using
Anti-Trimethyl-Histone H3 (Lys27) antibody, RM175.


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