A Peptide dotblot shows RM137 reacts only to Histone H3 trimethyl-Lysine 4 (K4me3). No cross reactivity with nonmodified Lysine 4 (H3N1-19), monomethylated Lysine 4 (K4me1) or dimethylated Lysine 4 (K4me2).
RM137 specifically reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross reactivity with other methylated lysines in Histone H3.
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM137 at 0.5 ug/mL, showed a band of histone H3 tri- methylated at Lysine 4 (K4me3) in HeLa cells.
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