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Anti-Trimethyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody, Clone RM155; H3K36me3, Histone H3 (trimethyl K36); ChIP Validated
品牌：RevMAb
貨號：31-1051-00
規(guī)格：100 ug
貨期：電詢
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Product Details
Product Name Anti-Trimethyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody [RM155]
Product Description Rabbit monoclonal to Trimethylated Histone H3 Lysine 36, H3K36me3, Histone H3 (trimethyl K36)
Catalog No. 31-1051-00
Clone Name RM155
Product Data Sheet Anti-Trimethyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody Data Sheet RM155
Specificity RM155 reacts to Histone H3 trimethylated at Lysine 36 (K36me3). No cross reactivity with monomethylated Lysine 36 (K36me1), dimethylated Lysine 36 (K36me2), or other methylations in histone H3.
Immunogen A trimethyl-peptide corresponding to trimethyl-Histone H3 (Lys36).
Application Western Blot (WB)
Chromatin IP (ChIP)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 ug/mL – 2 ug/mL;
ChIP: 2 ug/mL- 10 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords
.
Product Images
RM155

RM155 specifically reacts to Histone H3 trimethylated
 at Lysine 36 (K36me3). No cross reactivity with non-
 modified Lysine 36 (K36 Ctrl), monomethylated Lysine
 36 (K36me1) or dimethylated Lysine 36 (K36me2), or
 other methylations in Histone H3.

Western

Western Blot of recombinant histone H3.3 (1)
 and acid extracts of HeLa cells (2), using RM155 at
2.0 ug/mL, showed a band of histone H3
trimethylated at Lysine 36 (K36me3) in HeLa cells.

ChIP

ChIP performed on HeLa cells using H3K36me3 antibody
 (RM155, 5ug). Real-time PCR was performed using primers
specific to the gene indicated.


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