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Anti-Acetyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody, Clone RM154; H3K36ac, Histone H3 (K36 acetylation); ChIP Validated
品牌：RevMAb
貨號(hào)：31-1050-00
規(guī)格：100 ug
貨期：電詢
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Product Details
Product Name Anti-Acetyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody [RM154]
Product Description Rabbit monoclonal to Acetylated Histone H3 Lysine 36 (K36ac), H3K36ac, Histone H3 (K36 acetylation)
Catalog No. 31-1050-00
Clone Name RM154
Product Data Sheet Anti-Acetyl-Histone H3 Lys36 Rabbit Monoclonal Antibody Data Sheet RM154
Specificity RM154 reacts to Histone H3 acetylated at Lysine 36 (K36ac), and is not affected by the phosphorylation of neighboring Ser31. No cross reactivity with other acetylated Lysines in histone H3.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys36).
Application Western Blot (WB)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 ug/mL - 2 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords
.
Product Images
RM154

RM154 specifically reacts to Histone H3 acetylated
at Lysine 36 (K36ac), and is not affected by the
 phosphorylation of neighboring Ser31. No cross
 reactivity with acetylated Lysine 4 (K4ac), Lysine 9
 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac),
 Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 56
(K56ac), lysine 79 (K79ac), or Lysine 122 (K122)
 in histone H3.

Western

Western Blot of acid extracts from HeLa cells
untreated (-) or treated with sodium butyrate (+),
 using RM154 at 1.0 ug/mL, showed a band of
histone H3 acetylated at Lysine 36 in treated
 HeLa.

Immunocytochemistry

Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Acetyl-Histone H3 (Lys36)
Rabbit mAb RM154 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).


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