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Anti-Phospho-Histone H4 (pSer1) Rabbit Monoclonal Antibody, Clone RM194; H4S1p, Histone H4 (S1 phosphorylation)
品牌：RevMAb
貨號(hào)：31-1079-00
規(guī)格：100 ug
貨期：電詢
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Product Details
Product Name Anti-Phospho-Histone H4 (Ser1) Rabbit Monoclonal Antibody [RM194]
Product Description Rabbit monoclonal to Phospho-Histone H4 (Ser1), H4S1p, Histone H4 (S1 phosphorylation)
Catalog No. 31-1079-00
Clone Name RM194
Product Data Sheet Anti-Phospho-Histone H4 (Ser1) Rabbit Monoclonal Antibody Data Sheet RM194
Specificity RM194 reacts to Histone H4 phosphorylated at Serine 1. The reactivity is not affected by neighboring Arginine 3 modifications. No cross reactivity with Histone H2A phosphorylated at Serine 1.
Immunogen A phospho-peptide corresponding to the Phospho-Histone H4 (pSer1).
Application Western Blot (WB)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 ug/mL - 2 ug/mL;
ICC: 1 μg/mL - 2 μg/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 1 ug/mL.
Keywords
.
Product Images

Western Blot of (1) acid extracts of HeLa cells; (2)
recombinant Histone H4. Using RM194 at 0.5 ug/mL,
showed a band of Histone H4 phosphorylated at
Serine 1 in HeLa cells.

RM194

RM194 specifically reacts to Histone H4 phosphorylated
at Serine 1 (H4S1p). The reactivity is not affected by
neighboring Arginine 3 modifications (H4S1pR3me1
and H4S1pR3me2). No cross reactivity with Histone H2A
phosphorylated at Serine 1 (H2AS1p).

Immunocytochemistry

Immunocytochemistry of HeLa cells, using Anti-
Phospho-Histone H4 (Ser1) Rabbit mAb RM194
 (red). Actin filaments have been labeled with
 fluorescein phalloidin (green).


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