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上海拜力生物科技公司
Tel:400-968-7988    021-33779008
Anti-EGFRvIII Rabbit Monoclonal Antibody, Clone RM419
品牌：RevMAb
貨號：31-1305-02
規(guī)格：50 uL
貨期：電詢
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商品詳情 參考文獻(xiàn) 相關(guān)資料
Product Details
Product Name Anti-EGFR Variant III Rabbit Monoclonal Antibody [RM419]
Product Description Rabbit monoclonal to Epidermal Growth Factor Receptor Variant III
Catalog No. 31-1305-00
31-1305-02
Clone Name RM419
Product Data Sheet Anti-EGFR Variant III Rabbit Monoclonal Antibody Data Sheet RM419

Biotinylated Anti-EGFR Variant III Rabbit Monoclonal Antibody Data Sheet RM419
Specificity RM419 reacts to human Epidermal Growth Factor Receptor Variant III. No cross reactivity with wild type EGF receptor.
Immunogen A peptide corresponding to epidermal growth factor receptor variant III (EGFRvIII)
Application IHC, WB, ELISA, FC
Species Reactivity Human
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100 uL;
Biotinylated 50 uL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage IHC: 1:100 – 1:200 dilution;
FC: 1:10 – 1:100 dilution;
WB: 1:1000 - 1:2000 dilution;
ELISA: 1:100 – 1:1000 dilution.
Keywords
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Product Images
Immunohistochemical

Immunohistochemical staining of formalin fixed and paraffin embedded human Glioblastoma Multiforme tissue sections with or without EGFRvIIII using anti-EGFRvIII antibody (RM419) at 1:100 dilution.

Western

Western Blot analysis of DKMG (EGFRvIII positive), U118MG, A431, and HeLa whole cell lysates, using anti-EGFRvIII antibody (RM419) and anti-EGFRct (RM294) at 1:1000 dilution.

Flow

Flow cytometry analysis of DKMG and 293 cells, using anti-EGFRvIII antibody (RM419) at 1:50 dilution.

ELISA

ELISA analysis showing RM419 does not react to wild type EGFR protein. The plate was coated with recombinant EGFR variant III and EGFRwt proteins. A serial dilution of anti-EGFRvlll (RM419) or anti-EGFR (RM229) antibodies were used.

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