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Anti-Acetyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM149; H3K4ac, Histone H3 (K4 acetylation); ChIP Validated
品牌：RevMAb
貨號(hào)：31-1063-00
規(guī)格：100 ug
貨期：電詢
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Product Details
Product Name Anti-Acetyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [RM149]
Product Description Rabbit monoclonal to Acetylated Histone H3 Lysine 4 (K4ac), H3K4ac, Histone H3 (K4 acetylation)
Catalog No. 31-1063-00
Clone Name RM149
Product Data Sheet Anti-Acetyl-Histone H3 Lys4 Rabbit Monoclonal Antibody Data Sheet RM149
Specificity RM149 reacts to Histone H3 acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H3.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys4).
Application Western Blot (WB)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.25 ug/mL - 1 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.05 ug/mL – 0.5 ug/mL.
Keywords
.
Product Images
RM149

RM149 specifically reacts to Histone H3 acetylated
at Lysine 4 (K4ac). No cross reactivity with non-
modified Lysine 4, acetylated Lysine 9 (K9ac), Lysine
14 (K14ac), Lysine 18 (K18ac),Lysine 23 (K23ac),
Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56
 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in
histone H3.

Western

Western Blot of acid extracts from HeLa cells
untreated (-) or treated with sodium butyrate (+),
 using RM149 at 0.5 ug/mL, showed a band of
histone H3 acetylated at Lysine 4 in treated HeLa.

Immunocytochemistry

Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Acetyl-Histone H3 (Lys4)
Rabbit mAb RM149 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).


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