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Anti-Acetyl-Histone H4 (Lys8) Rabbit Monoclonal Antibody, Clone RM201; H4K8ac, Histone H4 (K8 acetylation); ChIP Validated
品牌：RevMAb
貨號(hào)：31-1082-00
規(guī)格：100 ug
貨期：電詢
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Product Details
Product Name Anti-Acetyl-Histone H4 (Lys8) Rabbit Monoclonal Antibody [RM201]
Product Description Rabbit monoclonal to Acetylated Histone H4 Lysine 8 (K8ac), H4K8ac, Histone H4 (K8 acetylation)
Catalog No. 31-1082-00
Clone Name RM201
Product Data Sheet Anti-Acetyl-Histone H4 Lys8 Rabbit Monoclonal Antibody Data Sheet RM201
Specificity RM201 reacts to Histone H4 acetylated at Lysine 8 (K8ac). No cross reactivity with other acetylated Lysines in Histone H4.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H4 (Lys8).
Application Western Blot (WB)
ELISA
Multiplex
Immunocytochemistry (ICC)
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.05 ug/mL – 1 ug/mL;
Immunocytochemistry: 0.5 ug/mL- 2 ug/mL.
Keywords
.
Product Images
RM201

RM201 specifically reacts to Histone H4 acetylated
at Lysine 8 (K8ac). No cross reactivity with unmodified
Lysine 8 (K8 ctrl), acetylated Lysine 5 (K5ac), Lysine
12 (K12ac), Lysine 16 (K16ac), Lysine 20 (K20ac),
Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.

Western

Western Blot of (1) acid extracts of HeLa cells treated
 with sodium butyrate, (2) acid extracts of HeLa cells
 untreated, and (3) recombinant Histone H4. Using RM201
 at 0.5 ug/mL, showed a band of Histone H4 acetylated
 at Lysine 8 in HeLa cells.

Immunocytochemistry

Immunocytochemical staining of HeLa cells treated
 with sodium butyrate, using anti-Acetyl-Histone
H4 (Lys8) Rabbit Monoclonal Antibody (clone
RM201) (red). Actin filaments have been labeled
with fluorescein phalloidin (green).


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