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ELISA of human immunoglobulins shows RM116
reacted to the γ1, γ2, γ3, γ4 heavy chain of human
IgGs, and the Fc of human IgG. No cross reactivity with
other human heavy chains, mouse IgG, rat IgG,
or goat IgG. The plate was coated with 50 ng/well
of different immunoglobulins. 200 ng/mL or 50 ng/mL
of RM116 was used as the primary antibody. An
alkaline phosphatase conjugated anti-rabbit IgG as the
secondary antibody.


Sandwich ELISA using RM116 as the capture antibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody (1ug/mL),
followed by an alkaline phosphatase conjugated streptavidin.


Cross-activity data of RevMAb anti-human IgG antibodies against
monkey IgG. ELISA plate was coated with 0.1ug/well (2ug/mL,
50uL/well) of Rhesus monkey IgG (protein A purified from serum).
A serial dilution of anti-human IgG (RM116), anti-human IgG1
(RM117), anti-human IgG2 (RM118), anti-human IgG3 RM119),
(anti-human IgG4 (RM120), anti-human kappa (RM126), and
anti-human lambda (RM127) was used as the primary antibody.
An alkaline phosphatase conjugated anti-rabbit IgG as the secondary
antibody.