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Detection of human IgG1 in monkey serum, using
RM117 (capture) and biotin-RM129 (detection)
as a Sandwich ELISA pair. HRP conjugated
streptavidin and TMB were used to yield the
colorimetric reaction.


ELISA showing RM129 reacts only to kappa and
lambda light chain of all human immunoglobulins,
not to mouse IgG, rat IgG, or goat IgG.


A titer Sandwich ELISA using Biotinylated RM129
as the detection antibody. The plate, coated
with the capture antibody anti-human IgG RM116,
was loaded with different amounts of human IgG.
A serial dilution of Biotin RM129 was used as
the detection antibody, followed by an alkaline
phosphatase conjugated streptavidin.