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Anti-Dimethyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody, Clone RM165; H3K14me2, Histone H3 (dimethyl K14)
品牌：RevMAb
貨號(hào)：31-1060-00
規(guī)格：100 ug
貨期：電詢
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Product Details
Product Name Anti-Dimethyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody [RM165]
Product Description Rabbit monoclonal to Dimethylated Histone H3 Lysine 14, H3K14me2, Histone H3 (dimethyl K14)
Catalog No. 31-1060-00
Clone Name RM165
Product Data Sheet Anti-Dimethyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody Data Sheet RM165
Specificity RM165 reacts to Histone H3 dimethylated at Lysine 14 (K14me2). No cross reactivity with monomethylated Lysine 14 (K14me1), trimethylated Lysine 14 (K14me3), or other methylations in histone H3.
Immunogen A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys14).
Application Western Blot (WB)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.25 ug/mL – 1 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords
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Product Images
RM165

RM165 specifically reacts to Histone H3 dimethylated
at Lysine 14 (K14me2). No cross reactivity with non
-modified Lysine 14 (H3K14), monomethylated
Lysine14 (K14me1), trimethylated Lysine 14
(K14me3), or other methylations in Histone H3.

Western

Western Blot of recombinant histone H3.3 (1)
 and acid extracts of HeLa cells (2), using RM165
 at 0.25 ug/mL, showed a band of histone H3
dimethylated at Lysine 14 (K14me2) in HeLa cells.


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