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Immunostaining of brain tumor tissue sections
using both EDTA and Citrate pretreatment methods.
Clinically validated to be Positive for the H3.3 K27M
Mutation by Sanger sequencing.


Image courtesy of Sebastian Brandner MD, Division
of Neuropathology and Dept. of Neurodegenerative Disease,
UCL Institute of Neurology, London, UK


Western Blot analysis of cell lysates prepared from
293T transfected with a DNA construct encoding
wild type (WT) or K27M mutant proteins of Histone
H3.3, using 0.01 ug/mL of anti-Histone H3 K27M
rabbit monoclonal antibody clone RM192.