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ELISA of mouse immunoglobulins shows RMG06
reacts to the Fc region of mouse IgG1, IgG2a, and
IgG2b, and very slightly to IgG3; no cross reactivity
with IgM, IgA, IgE, human IgG, rat IgG, and rabbit
IgG. The plate was coated with 50 ng/well of different
immunoglobulins. 500 ng/mL, 200 ng/mL, or 50
ng/mL of RMG06 was used as the primary antibody.
An alkaline phosphatase conjugated anti-goat IgG as
the secondary antibody.