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Anti-Acetyl-Histone H2.AZ (Lys4) Rabbit Monoclonal Antibody, Clone RM221
品牌：RevMAb
貨號：31-1101-00
規(guī)格：100 ug
貨期：電詢
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Product Details
Product Name Anti-Acetyl Histone H2A.Z (Lys4) Rabbit Monoclonal Antibody [RM221]
Product Description Rabbit monoclonal to Acetyl Histone H2A.Z (Lys4)
Catalog No. 31-1101-00
Clone Name RM221
Product Data Sheet Anti-Acetyl-Histone H2A.Z (Lys4) Rabbit Monoclonal Antibody Data Sheet RM221
Specificity RM221 reacts to Histone H2A.Z acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H2A
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H2A.Z (Lys4).
Application Western Blot (WB)
ELISA
Multiplex
Immunocytochemistry (ICC)
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 ug/mL – 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.05 ug/mL – 0.5 ug/mL;
Immunocytochemistry: 1 ug/mL- 2 ug/mL.
Keywords
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Product Images
RM221

RM221 specifically reacts to Histone H2A.Z acetylated
at Lysine 4 (K4ac). No cross reactivity with non-modified
Lysine 4 or other acetylated Lysines in histone H2A.

Western Blot of acid extracts from HeLa cells
 treated (+) or untreated (-) with sodium butyrate,
using RM221 at 0.5 ug/mL, showed a band of
histone H2A.Z acetylated at Lysine 4 in treated
HeLa.

Immunocytochemical

Immunocytochemical staining of HeLa cells treated
 with sodium butyrate, using anti-Acetyl-Histone
H2A.Z (Lys4) Rabbit Monoclonal Antibody (clone
RM221) (red). Actin filaments have been labeled
with fluorescein phalloidin (green).


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