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Anti-Acetyl-Histone H4 (Lys5) Rabbit Monoclonal Antibody, Clone RM199; H4K5ac, Histone H4 (K5 acetylation)
品牌：RevMAb
貨號：31-1081-00
規(guī)格：100 ug
貨期：電詢
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Product Details
Product Name Anti-Acetyl-Histone H4 (Lys5) Rabbit Monoclonal Antibody [RM199]
Product Description Rabbit monoclonal to Acetylated Histone H4 Lysine 5 (K5ac), H4K5ac, Histone H4 (K5 acetylation)
Catalog No. 31-1081-00
Clone Name RM199
Product Data Sheet Anti-Acetyl-Histone H4 Lys5 Rabbit Monoclonal Antibody Data Sheet RM199
Specificity RM199 reacts to Histone H4 acetylated at Lysine 5 (K5ac). No cross reactivity with other acetylated Lysines in Histone H4.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H4 (Lys5).
Application Western Blot (WB)
ELISA
Multiplex
Immunocytochemistry (ICC)
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.2 ug/mL - 1 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.05 ug/mL – 0.2 ug/mL;
Immunocytochemistry: 0.5 ug/mL- 2 ug/mL.
Keywords
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Product Images
RM199

RM199 specifically reacts to Histone H4 acetylated
at Lysine 5 (K5ac). No cross reactivity with unmodified
Lysine 5 (K5 ctrl), acetylated Lysine 8 (K8ac), Lysine
12 (K12ac), Lysine 16 (K16ac), Lysine 20 (K20ac),
Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.

Western

Western Blot of (1) acid extracts of HeLa cells; (2)
recombinant Histone H4. Using RM199 at 0.2 ug/mL,
showed a band of Histone H4 acetylated at Lysine 5
 in HeLa cells.

Immunocytochemistry

Immunocytochemical staining of HeLa cells treated
 with sodium butyrate, using anti-Acetyl-Histone
H4 (Lys5) Rabbit Monoclonal Antibody (clone
RM199) (red). Actin filaments have been labeled
with fluorescein phalloidin (green).


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