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上海拜力生物科技公司
Tel:400-968-7988    021-33779008
Anti-Mouse IgG2a Rabbit Monoclonal Antibody, Clone RM219
品牌：RevMAb
貨號(hào)：31-1098-00
規(guī)格：100ug
貨期：電詢
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Product Details
Product Name Anti-Mouse IgG2a Rabbit Monoclonal Antibody [RM219]
Product Description Rabbit monoclonal to Mouse IgG2a
Catalog No. 31-1098-00
Clone Name RM219
Product Data Sheet Anti-Mouse IgG2a Rabbit Monoclonal Antibody Data Sheet RM219
Specificity RM219 reacts to the Fc region of mouse IgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
Immunogen Mouse IgG2a
Application Western Blot (WB) (nonreduced only)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species Reactivity Mouse
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 0.005 ug/mL – 0.2 ug/mL;
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;
Western Blot (WB): 0.5 ug/mL-2 ug/mL.
Keywords
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Product Images
ELISA

ELISA of mouse immunoglobulins shows RM219
reacts to the Fc region of mouse IgG2a; no cross
reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA,
IgE, human IgG, or rat IgG. The plate was coated
with 50 ng/well of different immunoglobulins. 200
ng/mL, 50 ng/mL, or 10 ng/mL of RM219 was used
as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary
antibody.

A

A titer ELISA of mouse IgG2a. The plate was
coated with different amounts of mouse IgG2a. A
 serial dilution of RM219 was used as the primary
antibody. An alkaline phosphatase conjugated
anti-rabbit IgG as the secondary antibody.

Western

Western blot of nonreduced(-) and reduced(+)
mouse IgG2a, using 0.5ug/mL of RevMAb clone
RM219. This antibody reacts to nonreduced
IgG2a (~150 kDa).


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