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PathHunter® β-Arrestin2 Retroparticles enable the creation of stable β-arrestin2 cell lines in any cell type and the development of a β-arrestin2 assay for analyzing the activation of GPCRs, desensitization of most 7-transmembrane receptors, and regulation of other signaling molecules such as protein kinases. The retroparticles are Moloney murine leukemia retrovirus (MMLV) expressing β-arrestin2 fused to the β-galactosidase (β-gal) reporter fragment called EA (enzyme acceptor). The retroparticles can be used for effective transduction of a wide variety of dividing target cells (e.g. CHO, HEK293, U2OS) resulting in high–level expression of the encoded β-arrestin2-EA fusion protein. This product is intended for use with a plasmid expressing a ProLink?-tagged GPCR (or another β-arrestin binding protein) to create assays that report on β-arrestin binding or ligand-induced β-arrestin2 recruitment to the GPCR or β-arrestin binding protein of interest.

Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.