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上海拜力生物科技公司
Tel:400-968-7988    021-33779008
DiscoverX KILR Retroparticles for Adherent Suspension Cells (Hygromycin B) 97-0008
品牌:Eurofins DiscoverX
貨號(hào):97-0008
規(guī)格:97-0008
貨期:DiscoverX
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品牌:Discoverx 貨號(hào):97-0008 品名:KILR Retroparticles for Adherent Suspension Cells (Hygromycin B) 規(guī)格:4 vials KILR

商品詳情 參考文獻(xiàn) 相關(guān)資料

產(chǎn)品介紹

產(chǎn)品名稱

 KILR® Retroparticles for Suspension & Adherent Cells (Hygromycin B)

產(chǎn)品貨號(hào)

 97-0008

產(chǎn)品規(guī)格

 2 Vials x 0.5 mL (adherent cells), 2 Vials x 0.5 mL (suspension cells) 

 產(chǎn)品描述

 KILR® Retroparticles for Adherent and Suspension Cells (Hygromycin B) contains two types of MMLV particles, that can transduce adherent cells and suspension cells respectively. This leads to a high level expression with a housekeeping gene tagged with β-gal reporter fragment, ProLabel® (ePL) inside the target cells. Target cell death in a cytotoxicity assay results in the release of the ePL-tagged protein into the medium. Addition of KILR detection reagent, containing the complementing β-gal reporter fragment, EA, results in complementation of the two enzyme fragments (EA and ePL). The resulting functional enzyme hydrolyzes a substrate to generate a chemiluminescent signal.

冷凍標(biāo)簽

 KILR Retroparticles (Hygro) Adherent & KILR Retroparticles (Hygro) Suspension

產(chǎn)品詳情

 The KILR Retroparticles provide a potent vehicle for the stable delivery of the KILR reporter construct into almost any dividing cell line, enabling the creation of KILR cytotoxicity assays in the cell line expressing the target of your choice. The KILR Cytotoxicity cell line you generate enables the development of a simple, non-radioactive and dye-free method to specifically measure target cell death in a co-culture. The KILR assay has several applications in iOnc Cancer Immunotherapy (iOnc) drug development including measuring target cell death through Antibody Dependent Cellmediated Cytotoxicity (ADCC), Complement Dependent Cytotoxicity (CDC), Antibody Dependent Cell Phagocytosis (ADCP), cytotoxic T cell lymphocyte mediated death (CTL), Bi-specific Antibody Mediated T Cell Redirection, Chimeric Antigen Receptor T cell (CAR-T) and adoptive T cell therapies. This assay can drive all aspects of the drug discovery process, from screening to characterization, and the development of a QC lot release assay for the immunotherapy drugs.

KILR 細(xì)胞毒性試驗(yàn)的應(yīng)用

 

1. Antibody dependent cell-mediated cytotoxicity (ADCC)
2. Complement dependent cytotoxicity (CDC)
3. Antibody dependent cell phagocytosis (ADCP)
4. Cytotoxic t-cell lymphocyte mediated death (CTL)
5. Bi-specific antibody mediated T-cell redirection
6. Chimeric antigen receptor t-cell (CAR-T)
7. Adoptive T-cell therapies


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短期

 (<24 h): Store at -80°C 

長(zhǎng)期

 (>24 h): Store in vapor phase of liquid nitrogen.

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